Insecticidal Bioassay of the Organic Solvent-Extracted Chemical Fractions of Stinging Nettle (Urtica dioica) by Using Soybean Hairy Caterpillar (Spilarctia casigneta Kollar) as the Test Organism
Yubak D. G.C., R. C. Basnet, N. N. Khanal

An experiment was conducted at the Entomology laboratory of Institute of Agriculture and Animal Sciences (IAAS), Rampur in the winter of 2002/2003 with an objective to test the efficacy of nettle extracts to manage soybean hairy caterpillar (Spilarctina casigneta Kollar). A total of forty-two treatments including control (no nettle extract) and a positive check (pesticide) were evaluated for their deterrent properties to the leaf-eating caterpillar. Various extracts from the leaf of stinging nettle (Urtica dioca) was prepared at Central Bio-chemistry Laboratory of IAAS. Leaf discs of radish (Rahphanus sativus) having uniform size (1 mm 3) and weights were treated with various extracts. Second instar larvae of Spilarctia casigneta were allowed to take the treated leaf discs into 9 mm petriplates upon starving them until 12 hours. The result indicated that nearly 30% (out of forty two extracts) of the fractions extracted from chemical reagents such as chloroform, ethyl acetate and hexane are found to posses some sorts of antifeedant action to the larval development into the subsequent instars. It has further indicated the possibility of exploiting nettle extracts against biting and chewing pest insects indicating the ample opportunity of integrating them into the development of organic pest management.
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