Insecticidal Bioassay of the Organic Solvent-extracted Chemical Fractions of
Stinging Nettle (Urtica dioica) by Using Aphid (Brevicorinae brassicae)
as the Test Organism
Yubak D. G C., R. C. Basnet, N. N. Khanal

With an objective to test the efficacy of nettle extracts to aphid (Brevicorinae brassicae), an experiment was conducted at the Entomology laboratory of the Institute of Agriculture and Animal Sciences (IAAS), Rampur in the winter of 2002/2003. A total of sixteen treatments including control (no nettle extract) and a positive check (pesticide) were evaluated for their deterrent properties to the leaf-sucking insect. Extract from the nettle plant was taken from different parts such as leaf, stems, root with the use of organic solvents: ethyl acetate and hexane at 6000 and 3000 ppm concentration. Uniformly sized (1 mm3) leaf disc of broad leaf mustard (Brassica sp.) were treated with various extracts and starved nymphs of similar instar were allowed to take the treated leaf disc into 9 mm petriplates. The experiment indicated that some of the extracts derived from fresh leaves with ethyl acetate and hexane were able to inhibit the feeding behaviour of aphid. The extracts also affected the development of insect into subsequent stages indicating the possibility of exploiting nettle extracts against pest management.
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